Evaluation of the Dynamics of Morphophysiological Components during the Adventitious Roting of Eucalyptus nitens and Eucalyptus globulus

Author(s): María Paz Jofré, José Violido Becerra and Evelyn Daysi Bustos

There are many events that occur during adventitious rooting of cuttings from a physiological and morphological point of view. In the current study we evaluated cuttings of Eucalyptus nitens and Eucalyptus globulus to determine the physiological and morphological conditions differing rooting capacity. For thiswe induced rooting by exogenous applications of 4000ppm of indol butyric acid (IBA). After 0, 2, 5, 15 and 30 days of induction we obtained samples at the base of the cuttings for the morphoanatomic and phytochemical analysis (total phenols, flavonoids) and indol acetic acid (IAA). Morphoanatomical analysis shows that in both species root meristemoids formed from cells of the vascular cambium. During the rooting process there were differences between the two species on the measurements of phenolics compounds, E. nitens (800ug/gPF) had significantly higher values than E. globulus (200ug/gPF).Moreover, flavonoid compounds also differ between species, where rutin and quercetin were present only in E. nitens. This indicate that the phenolics compounds could make of difference. During the induction phase (first two days), IAA was in high concentrations, which decreased drastically during the following phases of adventitious rooting, this suggests a fundamental role in the induction phase of adventitious rhizogenesis.

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