Abstract

Combination Between Vitamin E and Calcium into the Neutralization of Novaluron in a Cellular Model of Fresh Water Paramecium spp.

Author(s): Rachid Rouabhi and Khadija Bazouzi

Most of researchers in toxicology regard just toxic effects of xenobiotic, whereas the detoxication and neutralization systems didnÂ’t get yet a value as objective of experimental studies. Chitin synthesis inhibitors (CSIs), pesticides of the third generation, formed a crucial matter for recent toxicological studies especially for BPUs, whose we choose the Novaluron as representative of this class of pesticides. Our work includes two sections, the first one concern the study of toxic effects of Novaluron on a eukaryote unicellular model Paramcium sp. As a non-target living specimen, the second section interested in the investigation about the neutralization capacity of the mixture Calcium/Vitamin E on the harmful effects of Novaluron on Paramcium cells. The evaluation of toxicity and neutralization are tested by two concentrations (10 and 20µg/ml) in strictly controlled conditions. Damaged effects of Novaluron are demonstrated by perforated cell walls of Paramecium sp. due to the inhibition of the Chitin synthesis and justified by neutral red coloration method, also Novaluron inhibits the growth of Paramecium sp cells in a dose-dependent manner, especially for the concentration (20µg/ml), in addition Novaluron increase response percentage, reduced number of generation, slowdown the speed of moving with hazardous path of swimming, biochemical analyses exhibit that Novaluron exhaust the cellular supply of glutathione. Calcium and Vitamin E combined with Novaluron reduce its destructive effects on the cell wall of Paramecium sp., but each of them by its mode of action, calciumas a controller agent of ciliary reversal and discharge of trichocysts by exocytosis, and Vitamin E as antioxidant agent who interferes in the detoxication system as an alternative agent of neutralization.