Abstract

Biochemical characterization of a respiration deficient mutant of aspergillus ochraecus

Author(s): Biswajit Ghosh, Abhishek Mukherjee, Syam Sundar Konar, Tapan Kumar Das

The sensitivity of intact mycelia of the respiration deficient mutant (rd1) towards cyanide and azide was found to be less compared with that of the wild type and also that of the cell free extract of the mutant; where as sensitivity of the cell free extract of the rd mutant was more or same as the wild type shown. Analysis of lipid composition of the rd mutant and the wild type indicated that total lipid aswell as individual lipids of the mutant were changed as compared with that of the wild type. The assay of succinate dehydrogenase and cytochrome oxidase activities in mitochondrial suspension of the respiration deficient mutant reflected that the mutant was respiratory incompetence.