Application of Ligninolytic Fungus in Decolourisation and Degradation of Some Textile Dyes

Author(s): Rina D Koyani and Kishore S Rajput

Plentiful production of the synthetic dyes is directly interrelated with the soil and water pollution. Therefore,to overcome this problem and its mitigation is always been serious and important issue worldwide.Application of microbial enzymes to remediate these recalcitrant compounds fromthe environment is very well-known biological solution, which is effective since last few decades. Present study deals with application of ligninolytic enzymes produced by a wild strain of Phanerochaete chrysosporium to degrade four reactive textile dyesviz. Reactive Red HE8B, Reactive Black B, Reactive Golden Yellow HR, and Reactive Violet 5R. The fungus was obtained from the decaying wood log of the tree growing Junagadh Forest of Gujarat State.It is not only proved to be potential strain in decolourisation of dyes but also proficiently degraded all the dyes under solid and liquid decolourisation media. The decolourisation and degradation study was efficiently supported by the FTIR analysis of dyes treated with ligninolytic enzymes produced by solid state fermentation.On plate ddecolourisation of dyes initiated after three days of fungal inoculation and the petri plates with 10 mg/L concentration of all four dyes were completely decolourised within 11 days, except Reactive Red HE8B which took 13 days after incubation. Supplementation of different sources of carbon and nitrogen influenced the process of decolourisation, in which dextrose and asparagines were found to be excellent supporters respectively.Interestingly one of the nitrogen sources i.e. sodium nitrite inhibited growth of the fungus.Influence of inoculum size on decolourisation was performed with different inocula size on solid and in liquid media, where one to three discs (10mm diameter agar plugs) were reported to be more effective in the rate of the decolourisation.

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