Aim: Many candidate genes for Chronic Obstructive Pulmonary Disease have been identified including the genes coding forMatrixmetalloproteinases, likeMMP9 andMMP12 which play an essential role in tissue remodelling and repair associated with COPD. The aim of the present study was to evaluate the association of 5A/6A promoter polymorphism of MMP3 gene with COPDfrom the South Indian population of Andhra Pradesh. Method: Two hundred and fiftyCOPDpatients and 250 controlswere included in the study. TheMMP3 gene 5A/6Apolymorphism was determined byAmplification Refractorymutation system(ARMS-PCR). Results:Asignificant difference was observed in genotypic frequency between patients and controls. The frequency of 5A/6A genotype was found to be significantly high in COPD in comparison with controls. Conclusion: TheMMP-3 promoter 5A/6A genotype is a risk factor for COPD Patients showing its involvement in the pathology of disease.