The present study describes the optimization of environmental conditions in colour removal by Pseudomonas oleovorans of an industrial textile dye. The first, a factorial design (2n-2) was performed to screen the most significant parameters and the second one, a full experimental design (24) was then carried out. Decolourization of the industrial dye was negatively influenced by tube volume and dye concentration, which is probably related to the maximal capacity of the strain to decolourize azo dyes. The decolourization process showed to be time-dependent. The best conditions for dye decolourization by Pseudomonas oleovorans based on the experimental design and economic factors were agitation speed, 0 RPM, pH8.5, temperature 32ï°C, 25% of tube volume, initial culture size of0.16 g·l-1, dye concentration of 50mg L-1. Under such conditions, a near total decolourization (95.4%) was achieved after 32 hours.