For many years, cultivars with narrow morphological deviations were evaluated using traditional field plot techniques. This technique is tedious and time consuming. Furthermore, the morphological characters do not guarantee the correct determination, because they are unstable and influenced by environmental conditions,. The advent of biochemical markers makes more exact identification possible. In addition, the biochemical techniques are rapid, accurate and dependable. The biochemical cultivar identification is useful to ensure the genetic purity of plant cultivars and their parental lines in commercial seed production of hybrids, aid for identification and characterization of diversity in crop cultivars and their wild varieties, analyze phylogenetic relationship of the accessions, asses the geographical origin of germplasm, describe a new cultivar, and speed up distinctness, uniformity, stability (DUS) test for candidate cultivar. It is also useful for the registration of new varieties, and plant variety rights applications. In this review, I made a survey for biochemical cultivar identification in the plant families: Poaceae, Leguminosae, Solanaceae, Compositea, Euphorbiaceae, Citraceae, Elaeagnaceae, Palmaea, Brassicaceae, Fagaceae, and Apiaceae in terms of the reproducibility of the techniques and their wide applications.