Abstract

Comparison of FBS and FBS substitute containing media in umbilical cord blood stem cell cultures: A preliminary study

Author(s): Jeanne Adiwinata Pawitan, Ecie Budiyanti, Arleni Bustami, Kanadi Sumapradja, Isabella Kurnia Liem

Aim: This study aimed to compare FBS and FBS substitute containing media in umbilical cord blood (UCB) derived stemcell culture. Experimental: Processing of UCB was done by Ficoll gradient or simple centrifugation, which was followed by lysis buffer treatment. The pellets were then cultured in FBS and FBS substitute containing media. Results:Attached cells were heterogeneous. There were fibroblastic cells and rounded cells of the macrophage-monocyte lineage. Ficoll gradient centrifugation and FBS containing DMEM, and simple centrifugation and VEGF and humanAB serum supplemented DMEMyielded confluent cultures. However, after passage-1, the cells differentiated predominantly into osteoclast-like cells, cell growth was arrested, and further passage was impossible. Conclusion: UCB is a poor source ofMSCs, therefore, Ficoll gradient and simple centrifugation processing combined with FBS or FBS substitute containing media are not suitable methods for the isolation and culture of UCB derivedMSCs.