Biochemistry & Molecular Biology Letters
ISSN (PRINT): 2454-9304

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Transformation Of F. Oxysporum

Agrobacterium tumefaciens-mediated transformation (ATMT) has long been used to transfer genes to a wide variety of plants and has also served as an efficient tool for insertional mutagenesis. This increased to over 80% when Agrobacterium tumefaciens cells grown in the absence of AS were used. Transformation of F. oxysporum was done with pCAMBIA 1302 vector harboring sGFP gene under the control of CaMV35S promoter and hygromycin B phosphotransferase (HPT) as the selection markerene (generously provided by Dr. Richard A. Jefferson, CAMBIA, Canberra, Australia). Two different protocols were followed- a) F. oxysporum spores were germinated in carboxy methyl cellulose (CMC) broth (15 g carboxymethyl cellulose, 1 g yeast extract, 0.5 gMgSO4, 1 g NH4NO3 and 1 g KH2PO4, for 1 litre broth) at 100 rpm, at28ºC for 1 week. The culture was filtered through two layers of cheesecloth and spores were harvested at 4,500 rpm, 4ºC for 5 min. Usinghaemocytometer, spores were counted and diluted to 106 spores/ml ofspore suspension. Agrobacterium-mediated transformation (AMT) method and, b)glass-bead method.

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Biochemistry & Molecular Biology Letters received 18 citations as per Google Scholar report

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