High Performance Liquid Chromatography Open Access Journals

 HPLC may be a highly improved sort of chromatography . A pump forces a solvent through a column under high pressures of up to 400 atmospheres. The column packing or adsorbent or stationary phase is usually a granular material made from solid particles like silica or polymers. The pressure makes the technique much faster compared to chromatography . this enables using much smaller particles for the column packing . The smaller particles have a way greater area for interactions between the stationary phase and therefore the molecules flowing past it. This leads to a way better separation of the components of the mixture. The pressurized liquid is usually a mix of solvents like water, acetonitrile and/or methanol and is mentioned because the mobile phase

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