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Serial Dilution Agar

In a serial dilution experiment for microorganisms which will grow on bacteriological media and become colonies, the most factors that affect the method are area of agar (dilution plate) during which the colonies grow and therefore the size of colonies being studied.A serial dilution may be a series of sequential dilutions wont to reduce a dense culture of cells to a more usable concentration. during this method, exactly 1 ml of every successive dilution is transferred into exactly 9 ml of liquid during a dilution blank, creating a 1/10 dilution. Serial dilutions are the culmination of variety of diluted tubes utilized in order to urge smaller dilutions. When a sample diluted 1/100 is added to a sample diluted 1/10, the ultimate dilution would be: (1/100) x (1/10) = 1/1000. the target of the serial dilution method is to estimate the concentration (number of colonies, organisms, bacteria, or viruses) of an unknown sample by counting the amount of colonies cultured from serial dilutions of the sample, then back track the measured counts to the unknown concentration.