Ultra Performance Liquid Chromatography and its Pharmaceutical Uses

Author(s): Abhishek Tripathi

Ultra-Performance Liquid Chromatography (UPLC) is a chromatographic system with a 1.7 m reverse-phase packing material and a pressure range of 6000-15000 psi (conventional HPLC employs 3 m - 5 m packing material and runs between 2000 psi and 4000 psi). Because of the reduced band broadening, the signal-to-noise ratio (S/N) is higher, and thus a climb in sensitivity. For complex mixture separation, this has resulted in improved chromatographic peak resolution, as well as increased speed and sensitivity. For a 10-minute separation, typical peak widths formed by UPLC are in the range of 1-2 seconds. The issue of ion suppression from coeluting peaks is greatly reduced thanks to UPLC's much improved chromatographic resolution. For analyzing complex mixtures, UPLC coupled to a Q-TOF mass spectrometer (Quadrupole Time-of-Flight Mass Spectrometry) and it is a powerful tool.

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