Abstract
simple reverse phase HPLC method for determination of partition coefficient of permethrin pesticide
Author(s): Tentu.Nageswara Rao, Tentu.Srinivasarao, Parvatamma.BotsaThe present study was conducted to determine the partition coefficient of permethrin. The log Pow was calculated for the permethrin from the calibration curve generated from log k versus log Pow (capacity factor) of seven known reference compounds. Reference compounds and test item k values were generated using an HPLC equipped with Phenomenox C18 column. The isocratic HPLC method used in the study consisted of a mobile phase of methanol/HPLC water (90:10) on a reverse phase Phenomenox C18 column. Since, test item resulted in two peak areas permethrin-P1 and permethrin-P2, when analysed in HPLC, the calculation of weighted average log Pow was adopted and the weighted average log Pow value of test item was found to be 5.88 ±0.001