Purification of Sweet Potato Starch Wastewater using Natural Biopolymers: Modeling and CharacterizationAuthor(s): K. Thirugnanasambandham
In this present study, an attempt was made to investigate the efficiency of chitosan to recover a protein from sweet potato starch wastewater under various operating conditions such as agitation time, initial pH, chitosan dose and settling time. Four factors three level Box-Behnken response surface design (BBD) coupled with response surface methodology (RSM) was used to modeling the protein recovery process. From the BBD results, second order polynomial model was developed and its adequacy was evaluated by pareto analysis of variance (ANOVA). 3-D response surface contour plots were developed from model and it was used to study the interactive effects of process variables on response. Optimum operating conditions were determined using desired function methodology and were found to be: agitation time of 9 min, initial pH of 5, chitosan dose of 0.8 g/l and settling time of 35 min. Under these conditions, 95% of protein was recoverd. Recovered protein was analyzed and it could be used as a livestock feed ingredients. Electrophoresis, SEM analysis also used and myosin heavy chain bands at approximately 200 KDa, actin bands at approximately 40 KDa are obtained.