Abstract

Acomparative study of different approaches for stability-indicating determination of tizanidine in presence of its oxidative degradation product

Author(s): KhalidA.M.Attia,MohamedW.I.Nassar,AshrafAbdel-Fattah

Five, simple, accurate, selective and sensitive methods were developed for the determination of tizanidine hydrochloride in presence of its oxidative degradation product without previous separation. Confirming this novel degradation way of tizanidine HCL by IR, 1H NMR and mass spectroscopy techniques. The first method is the first derivative technique, measure peak amplitudes of derivatized spectra at 293 nm. The second method is the first derivative of ratio spectra (1DD) which used for the determination of tizanidine HCL in presence of its degradation product at 224 nm. The third method is the ratio difference technique which depends upon measuring peak amplitudes of ratio spectra at 231 and 320 nm using 15 ug /ml degradate spectrum as a divisor. The fourth method is the mean centering of ratio spectra technique which depends upon centering data of ratios at 321 nm. The fifth method is the dual wavelength technique which depends upon measuring peak amplitudes of zero order spectra at 290 and 326 nm. The calibration graphs were linear in the range of (2.5 6 20 ug ml-1) for tizanidine HCL.


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