Fluorescein dye is a synthetic organic compound available as a dark orange/ red powder soluble in water and alcohol. Fluorescein dye has an absorptionmaximumat 494 nmand emissionmaximumof 521 nm(inwater), the color of its aqueous solution varies from green to orange. In this study, fluorescein dye has been synthesized by the condensation of phthalic anhydride with resorcinol through a novel route of microwave irradiation technique under solvent free condition in high yields within a period of minutes in the presence of zinc chloride via the Friedel-Crafts reaction. The light fastness of the dyes was found to depend on the mobility of electrons through conjugated system from donating electron (OH) to electron withdrawing in (COO-) afforded a good value of light fastness. The structure of the dye is characterized and confirmed by melting point, elemental analysis, infrared, ultraviolet-visible spectroscopy (UV/VIS) and nuclear magnetic resonance (1H-NMR) data. This dye was used for dyeing wool and nylon 6 inmicrowave. Dyeingwool and nylon 6 with the prepared fluorescein dye using microwave irradiation was investigated. Moreover fastness properties of the dyed fabrics were found to be better as comparable with conventional dyed fabrics which saving time, energy and decreasing liquor ratio in both synthesis of dye and its application to dye wool and nylon 6 were achieved.