Group behavior is one of the most important and common tasks in bioinformatics, however, it is still not clear that whether variation phenomenon exist in group behavior. The aim of this study is to find out the variation phenomenon in bioinformatics. In this study, factor analysis algorithm and variance analysis algorithm is used to calculate variation phenomenon of group behavior in bioinformatics. Cultural behavior is used as sample for simulations. After simulations, the significance of variation phenomenon has been found. This study can be directions for future bioinformatics research and development.