Polycystic ovary syndrome (PCO) is one of the most public endocrine disorders affecting women. Metanephrines are O-methylated metabolites of catecholamines. Measurements of plasma, normetanephrine and metanephrine, provide a sensitive test for diagnosis of pheochromocytoma in adults but have not been evaluated in women with PCOS.This study was carried out at Karbala hospital for gynecology and obstetrics,the Kamal AL-SamaraiHospital. Fortyfivewomen fulfilling the clinical and biochemical criteria for polycystic ovary syndrome and hyperinsulinemia were enrolled before using any drugs. In additionBodyMass Index (BMI), fasting insulin, fasting blood sugar and HOMAlevelswere performed. FSH, LH, prolactin, insulin, free metanephrines and epinephrine were measured by enzymelinked immunosorbent assay, fasting blood glucose were measured by colorimetric methods. There was a significant increase (p<0.001) in PCOS patients when compared to the control group in fasting serumprolactin, insulin, free metanephrines and Epinephrine. In conclusion,there was a significant an increase in freemetanephrine and Epinephrine inwomenwith PCOS before using any treatments. These results in the present study are shown the first time as our knowledge, these factors may be useful in following developments in insulin resistance in subjects with polycystic ovary syndrome. Further studies are needed to certify these factors in other populations with metformin treatment or with other insulin sensitizers or when treated with diet and exercise.