According to China’s actual conditions, which mean comparatively poor economic conditions of farmers and the weak infrastructure in countryside, the controller, with lower cost and better performance, has been developed in order to enhance the level of greenhouse production management and automation. The main design feature of this system is to gather the information of CO2 concentration, through the analysis and comparison between the collected parameters and system setting. When the collected parameters do not conform to the requirement, through opened or closed of the control module to achieve the automatic adjustment of CO2 concentration in greenhouse. At the same time, this system chooses the USB interface chip CH375B which supports Host way and Slave equipment way. Insert U disk in the USB port, we can take the information of CO2 concentration gatherer, collecting site, collecting time and so on for the form of TXT at any time, kept and extracted data conveniently, giving the staff bring greatly facilitate to take date in different environmental conditions.