Introduction: Thyroid hormones have many important biological functions in our body. One of the major function is control of the basal metabolic rate and calorigenesis. The influence of thyroid hormones on electrolytes has not been well established and the basic mechanisms are not clearly understood. Only fewer studies show the association between thyroid hormones and electrolyte. Thus, in this study our major goal was to find out the levels of electrolytes in hypothyroidism and correlation of electrolytes with TSH. Materials and Methods: Total 60 subjects were included in the study. All subjects were female. Out of which 30 cases were clinically diagnosed hypothyroid patients and remaining 30 were controls. Triiodothyronine (T3), Thyroxine (T4), and TSH were estimated by fully automated Beckman coulter Access-2 analyser. The electrolytes like Serum sodium, potassium and chlorides were measured by Ion Selective Electrodes. Statistical data analysis was performed by using SPSS 18.2 software. Results: The electrolytes serum sodium, potassium and chloride levels were significantly decreased in hypothyroid cases (p<0.05). However, Serum sodium and potassium levels displayed negative correlation with TSH and positive with serum chloride levels in hypothyroidism cases. Conclusion: The overall results of our study presented that the serum electrolytes levels were significantly imbalanced in cases of hypothyroidism. The hypothyroid patients should be regularly screened and checked for serum electrolytes to prevent further possible obstacles.