Abstract

Synthesis and characterization of chitosan nanoparticles as perspective drug delivery carrier: Study of their degradation, antibacterial activity and in-vitro blood compatibility behavior

Author(s): Hemlata Bundela, A.K. Bajpai, Vishal Bharadwaj

Chitosan-TPP nanoparticles have been prepared using ionotropic crosslinking method. FTIR results show characteristic peaks of functional groups present in the nanoparticles. XRD studies revealed about 44.6 % crystallinity of chitosan-TPP nanoparticle material. SEM and TEM studies show the spherical shape of the particles with 20-90 nmsize. Swelling ratio of the particles in PBS and SBFwas found to be in the range of 0.55-3.36 and 2.66- 4.19 respectively.%degradation of thematerial in PBS and in presence of enzyme papain has been calculated 46.6%and about 80.0%respectively. In-vitro blood protein adsorption for the nanoparticles was found to be in the range of 0.052-0.351mg/g and%haemolysiswas found to be in between 3.31- 42.1%.Antibacterial activity of thematerialwas checked against strain of E.Coli and Bacillus Cereus and percent inhibition of bacterial growth was found in the range of 11-35 %.