Bacteria of the genus Azospirillum are widely distributed in the rhizosphere of tropical and subtropical grasses and sugarcane. Azospirillum sp. is reported to occur in the rhizoplane of plants growing in saline soil. In this study the effect of Azospirillum on the growth and biochemical compound of Vigna mungo were analyzed Azospirillum biofertilizer was obtained from Tamilnadu Agricultural university, Coimbatore. The healthy Vigna mungo seed sample was purchased from local agro market at Thanjavur. Azospirillum was mixed with soil at different concentration, such as 1, 2, 3 and 4gm. Control wasmaintained without Azospirillum. The Vigna mungo seed coated with Azospirillum slurry and 10 seeds were sowed in each pot including control pot. After sowing the germinating ability, shoot and root length and leaf length and breath also calculated. After 25th day from the sowing the plant total chlorophyll and protein content was estimated. All the parameters increase in 4gm Azospirillum inoculated plant compared to other plants.