Three simple, precise and economical spectrophotometric methods have been developed for the estimation of granisetron in bulk and pharmaceutical preparations. Method A involves the determination of granisetron by dissolving in distilled water and followed by measuring the absorbance at 301 nm. Method B involves the derivatisation of the primary spectra to the second order. The drug obeyed Beer’s law in the concentration range of 5-50 µg/mL for both the methods. Method C is based on the reaction of granisetron with ferric chloride and 1,10-phenanthroline to form blood red coloured chromogen and followed by measuring the absorbance at 510 nm. The results of the analysis were validated statistically and found to be satisfactory.