Abstract

Spectrophotometric Estimation of Abacavir Sulphate in Pharmaceutical Formulations

Author(s): K. Naresh Kumar, N. Appala Raju and K. Mukkanti

 Three simple, accurate, rapid and sensitive methods have been developed for the estimation of abacavir sulphate in its pharmaceutical dosage form. The ninhydrin method is based on the formation of chloroform extractable complex of abacavir sulphate with ninhydrin to form a colored compound. The

ascorbic acid method is based on the formation of chloroform extractable complex of abacavir sulphate with ascorbic acid to form a purple colored compound. The PBQ Method is based on the formation of chloroform extractable complex of abacavir sulphate condensed with carbonyl group of PBQ to form a
chromogen. These Methods shows absorbance maxima at 580 nm, 535 nm, 395 nm and obeys the Beer’s law in the concentration range of 5-60 mcg/ml, 5-80 mcg/ml and 10-60 mcg/ml respectively. Results of analysis for all the methods were validated statistically and by recovery studies. The proposed methods
are economical and sensitive for the estimation of abacavir sulphate in bulk drug and in its tablet dosage form.

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