All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Simultaneous HPTLC determination of Camylofin dihydrochloride in pharmaceutical preparations

Author(s): P.S.Barde, A.Y.Desai, Shikha M.N.Roy, V.V.Vaidya

A simple, fast and precise high performance thin layer chromatographic method has been developed for the simultaneous determination ofCamylofin dihydrochloride usingMebeverine as an internal standard. Chromatographic separation of these two drugs was performed using Silica gel 60F 254 as stationary phase with a mobile phase comprising of Benzene:Methanol and Ammonia (75:25:3 v/v). Under these conditions the Rf for Camylofin peak was 0.62. Quantitation was achieved by densitometric scanning at 254nm. The Rf values for Camylofin dihydrochloride and Mebeverine HCl peaks were 0.62 and 0.78 respectively. The proposed method was validated for linearity, accuracy, precision. The response to Camylofin dihydrochloride was a linear function of concentration over the range 125-1000 µg mL-1 i.e. 25% to 200% of the working assay concentration. The method permits reliable quantification of Camylofin dihydrochloride and good resolution and separation of Camylofin dihydrochloride from internal standard of Mebeverine HCl. It can be conveniently adopted for routine quality control analysis.


Share this       
Awards Nomination 20+ Million Readerbase

Table of Contents

Google Scholar citation report
Citations : 378

Analytical Chemistry: An Indian Journal received 378 citations as per Google Scholar report

Indexed In

  • CASS
  • Google Scholar
  • Open J Gate
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Cosmos IF
  • Electronic Journals Library
  • Directory of Research Journal Indexing (DRJI)
  • Secret Search Engine Labs
  • ICMJE

View More

Flyer