Abstract

Separation and Identification of the Components of Acacia Sieberiana Stem Bark Plant Extract and Study its Safety as a Treatment Drug for Diarrhea

Author(s): Elmezian H, Zawawy FM, EL-Helbawy S, Mostafa M, El Aily M and Ayat Mosleh

The Safety of the stem bark of Acacia Siberian, as an antidiarrheal, in Albino rats (four groups) has not yet been evaluated. This study to identify the effect of the acetone extract of the stem bark of Acacia Siberian (AESBAS) on Albino rats, Separation and identification of the acetone extract of the stem bark of Acacia Siberian by GC-MS-TMS analysis. Albino rats (four groups) were subjected to the sub-chronic oral administration of the AESBAS for Twenty-eight days. Phytochemical screening test of the AESBAS confirmed by GC-MS-TMS analysis. Non-significant effect (p>0.05) in mean weight gain during the end of the experiment between groups. Also, some hematological analysis in the twenty-ninth day was estimated using standard steps. The AESBAS had non-significant effect (p>0.05) on some hematological analysis on study groups compared with the control group, that were given varying doses of the AESBAS. The components of AESBAS confirmed by GC-MS-TMS analysis. The results of this study suggest the AESBAS does not possess haemotoxic activities that could limit therapeutic use as an antidiarrheal. Phytochemical screening of the AESBAS by GC-MS-TMS analysis, confirms the existence of various compounds with different chemical structures, analysis of the AESBAS confirms the results data tests for phytochemical screening.