Bacillus licheniformis levansucrase was immobilized on different carriers using different immobilization methods including physical adsorption, covalent binding, ionic binding and entrapment. The most efficient immobilization was achieved by chitosan (10%) using glutaraldehyde as a bifunctional agent. Thismethod gave an immobilization yield of 83%and a levansucrase activity recovery of 97%. The immobilized enzyme exhibited a shift in the optimal pHfrom5.5 to 7.0, but the optimal temperature of activity was not affected. The immobilized enzyme retained about 50%of its initial catalytic activity even after being used during 5 successive uses. The main product synthesized was levan which could be used for important applications.