Attractive properties of gelatin, such as good biocompatibility, low immunogenicity, plasticity, adhesiveness, promotion of cell adhesion and growth, and lowcost, make it ideally suitable as a biomaterial for tissue engineering. Highly volatile solution of gelatin (gel-PEG-DEE) and polyethylene glycol (PEG) in diethyl ether (DEE) was prepared for wound healing applications. The compatibility of gel-PEG-DEE with blood sample was performed that results demonstrated that the substance was fully compitable with human blood sample. In addition to this gel-PEG-DEE also showed antibacterial properties with no cytotoxicity as observed by brine shrimps analysis. The solidification time of the solution was found to be 4 sec which is extremely good as wound healing gel. The in vivo wound healing characteristics of the gel-PEG-DEE (highly volatile solution) was evaluated using rat (Rattus norvegicus) model test. Full-thickness wounds were created on the dorsal surface of the Rattus norvegicus and were treated with both gel-PEG-DEE and Âeco-plasterÂ (control and commercially available wound healing materials) to evaluate the efficiency of the gel. The characteristic results revealed that the gel-PEG-DEE gelatinous solution was found to be more effective in the healing of rat wound than the commercial eco-plast.