Drugs derived from plants are gaining new prospects as traditional antibiotics are becoming ineffective. This study was to screen the bioactive phytochemicals of Cyperus pangorei because biological screening of the plant was very limited and it was recently found to be used as a substitute to C.rotundus which is known to have many bioactive components. Quantitative analysis was by extracting and estimating the compounds by UV spectroscopy and other techniques and qualitative analysis was by thin layer chromatography. Since there was no available literature on the cytotoxic effects of the plant, the study also focused on cytotoxicity screening of the plant extracts. Vero cell lines and human lung carcinoma cell lines were used for this purpose. C. pangorei possessed several primary and secondary metabolites and the crude extract was not cytotoxic.