The present study was aimed to evaluate the anti-inflammatory properties of seed extracts of Annona squamosa in rats. The ethanol extract of Annona squamosa was extracted by using Soxhlet extraction apparatus. The preliminary phytochemical studies revealed for the presence of alkaloids, reducing sugar, fixed oil and fats, phenolic compounds and tannins, proteins and amino acids. The antiinflammatory profile of this extracts was investigated on the basis of paw edema induced by carrageenan. The extract (100 mg/kg) caused a maximum inhibitory activity on compared with standard reference drug indomethacin. The ethanolic seed extract of A. squamosa significantly (p < 0.001) inhibits the inflammation of about 36.33% in paw edema induced by carrageenan and indomethacin (10 mg/kg) inhibits about 35.13%. On the basis of these findings, it may be inferred that A. squamosa exerts anti-inflammatory activity at the second phase of carrageenan inflammation.