Many attempts weremade to improve roughness of titaniumimplants which is important for early fixation and long-term mechanical stability of the prosthesis. In the present work microstructure, surface roughness and hardness of titaniumbiomaterialwere investigated before and after exposure to magnetic resonance imaging (MRI) and gamma radiation. It was found that microstructure, (crystallinty, crystal size and orientation), of titanium biomaterial was changed after exposure to magnetic resonance imaging (MRI) and gamma radiation. Roughness parameter (Ra) was significantly increased after exposure to magnetic resonance imaging and gamma radiation. However, Vickers hardness number of titaniumwas significantly decreased.