Samadera indica (Gaertn) is locally known as Lakhandi. This Plant belongs to natural order Simarubaceae. Its bark is useful for curing fever. Its leaves when bruised is applied for curing insect bites. Its kernels are used for treating rheumatism. In the present study, we describe the isolation and structural elucidation of the flavone-o-glycoside, luteoline-7-o-β-D-glucopyranoside isolated from the kerlels of Samadera indica (Gaertn).