In this review article we have out lined the applications of Laser in Biotechnology. The laser-based systems are the state of art technologies for distinct and noncontact manipulations in cellular or sub-cellular dimensions with nanometre accuracy and have become fundamental tools within entire community of life science research,. Laser-induced optical forces can be used to guide and deposit 100nm-10µm diameter particles with micrometer accuracy; this process is known as Âlaser-guided direct writing Â. Laser and its scrupulous application to various steps in IVF (In-Vitro Fertilization) process is a field of growing interest. The diversity of compact blue-green lasers in both the variety of technical approaches used to produce them and the wide range of applications for which they have been sought has made the field of compact blue green lasers interesting. Single cellmanipulation in tissue is performed with the use of shockwave manipulation and laser trapping. The laser method is used to improve gene transfer for therapeutic purposes.