Food products are the ideal sources of nutrition for microorganisms. Food deterioration and spoilage may occur when microorganisms naturally contaminating the food articles are allowed to grow beyond certain limits. Four types of bacterial strains were isolated from Brassica oleracea L. and biochemically characterized. They were identified as E.coli, E. aerogenes, Staphylococcus aureus and Bacillus sp. Antimicrobial activity of ayurvedic formulations namely Hajmola (Dabur), Pudin Hara (Dabur), Ginkgo biloba (Bilovas), Shankhvati (Zandu) were screened against the isolated bacterial strains. MIC and MBC were also tested. MIC obtained between the range of 3.12–25mg/ml. MBC was found between the range of 12.5 to >50 mg/ml. Pudin hara was found to be most potent as all the tested bacterial strains were inhibited by it. Hajmola was the second best drug that exhibited good antibacterial activity against E.aerogenes and E.coli.