Pseudomonas aeruginosa is an important agent of nosocomial infections. In the present investigation incidence of P.aeruginosa in various clinicalmanifestations in Gulbarga hospital settings is investigated. A total of 357 isolates of P.aeruginosa were obtained from 1026 non duplicate samples accounting to an incidence of 34.79%. P.aeruginosa displayed uniform biochemical activities. Highest incidence of P.aeruginosa was found in burn wounds (77.77%), followed bycellulitis (67.18%), diabetic foot ulcer (55.85%), urine (43.14%), openwounds (36.92%) and ear swabs (12.3%) and lowest in anterior nares (1.6%) and Blood (5.12%) samples. Incidence of gender wise distribution showed no significant variations. High prevalence of P.aeruginosa infection occurred in the age group of 20-29 years and least in >80years of age group.