Based on the prevailing traditional knowledge at Hyderabad Karnataka region, Caralluma adscendens var.adscendens was selected for the present studies and extracted the plant materials in different solvents like, methanol, ethanol, ethyl acetate, hexane and water. These different solvent extracts were tested against seven clinical bacterial isolates viz, Vibrio cholerae, Shigella dysentriae Type1, Salmonella paratyphi B, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae and Escherichia coli. Ethyl acetate extract showed significant inhibitory activity among the solvent extracts tested,while water extract has negligible activity against the pathogens. The root extracts in all the solvents including aerial part extract in hexane did not show any activity. The ethyl acetate aerial part extract at 80mg/ml concentration showed higher activity against most of the bacterial isolates and similar and even higher activity than penicillin and ampicillin at 10mg/ml. The standard ciprofloxacin hadmore significant inhibitory activity compared to other extract and standards.