Abstract

In Vitro Cytotoxic, In Vitro and In Vivo Antidiabetic Activity of Roylea Cineria

Author(s): R. P. Bahuguna, J. S. Jangwan, R. Singh and U. P. Bhatt

Roylea cineria belong to family Lamiaceae is an evergreen, erect or scandent aromatic shrub found at 2000-5000 ft high. It is commonly known as Karui. In vitro cytotoxic activity of ethanol extract was carried out against different cell lines SK-Mel 41, U-87 MG, Hela, MDA-MBA-231 cell line and LC50 value was determined was found to be 131.8 μg/mL, 275.4 μg/mL, 302.0 μg/mL, respectively. In vitro anti-diabetic potential of EtOH extract (UB-1), petroleum ether extract (UB-2), ethyl acetate extract (UB-3), MeOH extract (UB-4) was tested, which showed maximum of 65.7%, 39.0%, 42.9% and 61.3% PTP-1B% inhibition, respectively at 100 μM concentration. IC50 value for UB-1 and UB-4 was found to be 7.5 μm and 9.1 μM, respectively. In vivo anti-diabetic potential of EtOH extract (UB-1), petroleum ether extract (UB-2), ethyl acetate (UB-3), MeOH extract (UB-4) were tested for lowering the blood glucose level of streptozotocin-induced diabetic rats. UB-2, UB-3 and UB-4, which showed decline in blood glucose level to the tune of 23.4% (p < 0.01), 18.2% (p < 0.01) and 20.7% (p < 0.01) during 0-24 hr, respectively at 100 mg/Kg oral dose


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