In the present study, in vitro anticancer activity of ethanolic extracts of leaves and stems of Peristrophe Bicaliculata, Nees were evaluated. In vitro anticancer activity was assayed by tryphan blue dye exclusion method and brine shrimp lethality bioassay method. Extracts were found to be cytotoxic in a dose dependent manner in both methods. The CTC50 in leaves is 165 μg/mL and in stems is 219 μg/mL in EAC cell lines. The LC50 in leaves is 173 μg/mL and in stems is 207 μg/mL in brine shrimps. Concentration needed for 50% inhibition was found to be less in leaves as compared to stems. It indicates that Peristrophe Bicaliculata, Nees has potential to prevent cancer in a dose dependent manner.