Identification, Purification and Quantification of Andrographolide from Andrographis Paniculata (Burm. F.) Nees by HPTLC at Different Stages of Life Cycle of Crop

Author(s): Meenu Sharma and R. G. Sharma

A simple, rapid, selective and quantitative HPTLC method has been developed for determination of Andrographolide in Andrographis paniculata at different stages of life cycle of crop. Whole plant material at different stages, from 30 days of plantation up to maturity of the crop was studied. Chromatogram scanned at 250 nm. The results observed under UV light showed a good separation for all compounds. The spots for Andrographolide in samples were confirmed by comparing the Rf and spectra of the spot with that of standard biomarker of Andrographolide. Rf for Andrographolide was found to be 0.31. The average Andrographolide content varied from 0.42% to 2.02% in the sample studied.

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