A high performance thin layer chromatography (HPTLC) method was developed and validated for quantitative determination of cilostazol. The analysis was performed using a mobile phase composed of acetate : toluene : methanol : ether (2 : 2 : 1 : 0.5 v/v). The calibration graphs were linear (correlation coefficient (r) = 0.997) in the studied concentration range of 100 to 800 ng/mL for cilostazole in HPTLC. Relative standard deviation of all the parameters for intraday and interday precision is 0.75 to 2 and the accuracy was greater than 98%. The limit of quantification of the drug was found to be 20.5 ng/spot and the limit of detection of the drug was found to be 6.765 ng/spot. So the proposed method is precise and accurate and can be applied directly and easily to the cilostazol.