The inflammation of the conjunctiva is one of the common ocular disorders, which present redness, watering of the eye and conjunctival congestion and is most frequently occurring in the developing nations. Herbal eye drops are cheap, reliable and have little side effects than the costly synthetic drugs. In the present study, the herbal eye drop formulation was prepared and investigated for its anti-inflammatory (rabbits) and antiallergic activity (guinea-pig ileum), using in vivo and in vitro experimental models, respectively. The data was statistically analyzed using one-way analysis of variance (ANOVA) method. The minimum level of significant was fixed at p < 0.01 and p < 0.05. The present study was undertaken to elucidate the role of this herbal product in a variety of infective, inflammatory and allergic disorders.