Niosomes are nonionic surfactant vesicles that have potential applications in the delivery of hydrophobic and hydrophilic drugs. Niosomes have been prepared with different surfactants. Different batches of metoprolol tartrate niosomes were prepared by changing the surfactant concentration but keeping the cholesterol concentration constant. The prepared niosomes were characterized for particle size, entrapment efficiency and drug release studies. It was observed that Span 60 based formulations have higher entrapment efficiency than other formulations. Also Span 80 based formulations produced vesicles of smallest size and maximum cumulative percent drug release. In conclusion, the niosomal formulation could be a promising delivery system for metoprolol tartrate.