In this study, extraction of Chamaecyparis obtusa oil from leaf, wood, bark, cone using supercritical CO2 was carried out. The highest yield was obtained from leaves. The supercritical CO2 extraction of C. obtusa was influenced by temperature. When the temperature increased from 40℃ to 50℃ and 60℃, the yield increased. Among various parts of C. obtusa, the cone showed higher temperature dependency on extraction yield and rate than other parts. The color of oils extracted was different. The oil color from leaves was deep green. The oil from wood was brown red. Oil colors from bark and cone were yellow and brown respectively.