The Benincasa hispida fruit was extracted with solvent like methanol and water. The extractswere studied for both antibacterial and antifungal activity. The extracts did not show any antibacterial activity but it has remarkable antifungal activity against Aspergillus niger and Candida albicans. The methanolic extract was subjected to column chromatography, chloroform and ether fraction (40:60) produced crystals. The isolated crystals (Inositol) were confirmed through characterization such as IR, 1H NMR and chemical test.