Five efficient amylase producing thermophiles were isolated and characterized fromhot spring ofUnkeshwar,DistrictNanded,Maharashtra. Of these, a gram positive rod shaped organism APP-1 was selected for amylase production. It was identified as Paenibacillus alvei using cultural, microscopic and biochemical characterization. Isolate APP-1 showed luxuriant growth and amylase production at temperature 55oC and pH 7.0. SDS-PAGE analysis revealed molecular weight of amylase was 65 kDa. Amylase was found to be stable in presence of commercially available detergents. Catalytic activity of amylase was enhanced in presence of 1mM CaCl2 and 2-Mercaptoethanol.