Evaluation of the genotoxic properties of dibenzo sulfide (DBS) and dibenzo sulfoxide (DBSO) macrocyclic diamides (DSOMD) in the human lung cell line A549Author(s): Masoud Mashhadi Akbar Boojar, Abbas shockravi, Farzan Mashhadi Akbar Boojar
Background:Macrocyclic diamides are derivatives of two Tri-Aza macrocyclic crown ethers, poses the ability to transport across membrane and interfere with different living systems. The genotoxic potential along with cytotoxicity of dibenzo sulfoxide (DBSO) and dibenzo sulfide (DBS)macrocyclic diamides were comparatively checked by various biochemical tests inA549 cell line. Methods: We assessed the effects of these substances in a range doses (0.1 to 8 mMol) on ROS level, cellular viability, colony efficiency, micronucleus (MN), comet test and on HPRT gene mutation. Results: Both compounds revealed cytotoxicity effects on cell culture particularly at doses >1 mMol after 24 h incubation. They decrease colony efficiency and cellular viability of cells and significantly promoted ROS generation. There were also marked increase in; MN frequencies, DNA migration in comet assay and genemutation induction at the HPRT locus, in which DBSOMD was more effective. Genotoxicity appeared at concentrations that were cytotoxic. Conclusion:We documented ROS generation potential of the studied crown ethers and their possible consequence cytotoxicity. We also presented indications of genotoxicity effects through DNA damages.