Evaluation of changes in enzyme activities and nutrient degradation of chickpea seed (Cicer arietinum L.) during germinationAuthor(s): M.Shaela Pervin, M.G.Sarowar Jahan
Enzyme activities and mobilization of seed storage substances were investigated in BARI-4, BARI-5, and BARI-6 varieties ofChickpea seed (Cicer arietinum L.) at different germination periods. Among the varieties, the highest urease activity was found in BARI-4 and lowest in BARI-5 during germination at 192 hours in water. Of the varieties, BARI-4 contained the highest and BARI-6 contained the lowest amylase activity during 144 hours. Among the varieties, themaximumactivity of invertase was found in BARI- 5 andminimuminBARI-6 at 144 hours of germination. The activity of lipase was highest in BARI-4 and lowest in BARI-5. The highest protease activity was found in BARI-5 and lowest in BARI-4. The amount of total protein and water soluble proteinwere found to be highest in BARI-5 and lowest in BARI-4. During germination, protein depletion starts after initial imbibitions, and is completed in 240-288 hours. The varietyBARI-4 was found to contain the highest amount of free sugar while BARI-6 contained the lowest amount. Of the varieties, BARI-5 was found to contain the highest amount of reducing sugar and BARI-4 contained the lowest amount. The highest amount of starch was found in BARI-6 and lowest in BARI-4. The starch content in chickpea seed decreased gradually during germination. Among the varieties the BARI-4 was found to contain the highest amount of lipid while the BARI-5 contained the lowest amount. The seed storage substances decreased gradually with the increase of germination time. The results suggest that degradation of seed reserve nutrients accelerate the development of seedling growth during germination.