A peptic ulcer is an area of the gastrointestinal tract that is usually acidity and thus, extremely painful. For this propose, the healing effect of poly herbal formulation containing the combination of four plants viz. Piper betel, Emblica officinalis, Ocimum sanctum and Azadiracta indica were evaluated on indomethacin induced gastric ulceration in rats. 40 Rats were divided into 8 groups; 5 animals in each groups. Groups I rats serving as the ulcerated control received only indomethacin and were sacrificed four hours after indomethacin administration. Ulceration in the groups II-VIII rats was induced with indomethacin dissolved in distilled water. Group II received only the vehicle oral dose of gum acacia in distilled water (1 mL per rat). Group III-VII rats received the only the poly herbal formulation. Group VIII rats received misoprostal (1.43 μg body weight) once daily by oral intubation starting from four hours after the indomethacin administration. After seven days, the rats of groups II to VIII were sacrificed by cervical dislocation followed by cutting of abdominal aortic artery. The ulcer activity of poly herbal formulation was evaluated by total acidity and free acidity.