The aimof this research is to explore the effects of starvation and refeeding on the digestive enzyme activity of Megalobrama pellegrini, to find out its regularity and enrich its starving physiology. In this experiment, Megalobrama pellegrini were divided into five groups, as starved for 0(control), 2, 4, 7, 14day and refed for 14daywith enough food. The specific activities of protease, lipase and amylase were determined during starvation and refeeding. The results showed that effects of different level of protease, lipase and amylase were produced by starvation. Along with starved time extension, the activities of protease, lipase and amylase overall presented downward trend, and the activity presented stabilized in the late starvation. The amylase and lipase activity of intestinal significantly decreased at first and then after a slight downward trend in rising. The specific activities of protease, lipase and amylase were increased rapidly after refeeding, and reached a peak when refeed two days. As continue refeeding the activity gradually recovered to the level before starvation.