Abstract

Dried Powder of Unani and Ayurvedic Medicines Yields Remarkable DNA upon Extraction

Author(s): Nishat C, Shingo U, Farhad Hossain MD, Saiful Hoque MD, Linkon K and Akter L

Genomic DNA extraction is an important step in plant molecular biological research. The objective of the study was to recommend the customized genomic DNA extraction method for some kobiraji medicines in Bangladesh. The customized plant genomic DNA extraction method extracted eight different ayurvedic medicine powders such as Phyllanthus emblica, Withania somnifera, Abroma augusta, Syzygium cumini, Oroxylum indicum, Chrysogenum aurium, Mucuna pruriens and Terminalia bellirica. The quantity of the extracted genomic DNA was measured and tested at 260 nm using Nanodrop® ND-1000 spectrophotometer and the quality of DNA was determined by the horizontal agarose gel electrophoresis using 1% agarose in TBE buffer at constant voltage of 60 V. Genomic DNA extraction gave original and real DNA with amplifiable and usable amount for all the powder medicine tested. Sufficient amount of DNA was obtained from the powder medicines, but no result found in electrophoresis as the DNA size was too large.